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Int. J. Nat. Soc. Sci., Research Report
Web Release Date: Mar. 22, 2009 Copyright © 2008-2009 International
Association of Natural and Social Science
Free and Glycosylated Emodin in Caulis Polygoni multiflori
Mingguo Lia, YingqinWeib*,Peihai Weic, Biqi Lid, Fang Hai-yaib / Shuxiang Wange
a School of Life Science,Shihezi University, Shihezi 832003 China, E-mail address:limingguo2006@yahoo.com.cn
b School of Chemical Engineering, Shandong Institute of Light Industry; Jinan, Shandong, 250100, China, E-mail address: wyqtcm@yahoo.com.cn
c Department of Chemistry; Shandong Institute of Education; Jinan 250013, China
d School of Chemistry and Chemistry Engineering,Shihezi University,Shihezi,832003,China
e School hospital of Shandong Institute of Light Industry; Jinan, Shandong, 250100, China
Received Mar. 22, 2009
Accepted May 1, 2009
Abstract:
Content of free and glycosylated emodin in Caulis Polygoni multiflori was determined in this paper. HPLC was used with C18 column (250 mm x 4.6 mm, 5?m), methanol with 0.4% phosphoric acid solution (85.5?14.5, V/V)) as mobile phase and the detective wavelength was set at 254nm. The speed is 1.0 ml•min-1. Emodin and rhein in the samples were well separated. The content of emodin was linear in the range of 6.5-52 ?g ml-1?r=0.9994?. The average recoveries for determining free and glycosylated emo-din were 97.2-99.7% (R.S.D %, 0.99-1.28%) and 95.7-96.4% (R.S.D %, 0.82-1.39%) respectively. The content of free and glycosylated emodin was 0.43 and 0.19 mg g -1 respectively. The content of rhein was very low, free rhein was not detected while the estimated value of glycosylated rhein was 11.38 ?g g -1. Caulis Polygoni multiflori was usually doceted with water in clinical application, so emodin and glycosylated emodin or emodin and the total emodin as index are more scientific and comprehensive for the quality control of Caulis Polygoni multiflori.
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